MICROBIOLOGICAL PROCESS FOR CAPTURING VOLATILE COMPOUNDS AND PRODUCING PROTEIN BIOPOLYMER

Abstract

Coastal environments contaminated by oil show low recovery efficiency with traditional physical-chemical methods. As a solution, oil-contaminated mangrove fungus is isolated and a bioremediation process is carried out to degrade the hydrocarbons. A consortium of raw glycerin and mamona cake was used as a biostimulant, associated with the fungi Aspergillus spp and Penicillium spp in the fermentation process. This was carried out in field aquariums, containing samples inside for armazenagem of the compost samples, removed from the contaminated manguezal substrate; These forams are collected, quantified, homogenized and used to compose the microbial mixture. In this way, the consortium test was carried out on the composition (w/w) manguezal substrate contaminated with exuded oil; crude glycerin; mamona cake; production water, plus the addition of fungal strains. Obtained after 20 days at room temperature in the production of gelatinous biopolymers, the samples were initially analyzed in high efficiency liquid chromatography (HPLC) to identify the reference target of manguezal sediment, calcined sand, crude glycerin and mamona cake. Subsequently, the samples were analyzed using Rayos-X Fluorescence (inorganic) and Infravermelho FT-IR (organic) or manguezal sediment, reference, and the gelatinous biopolymer product. With two mineral elements given, a Covariant Statistical Analysis of Data – PCA is obtained, aiming at the identification of two products in biochemical transformation. An analysis by non-infrared spectroscopy showed evidence of bands in the region of 1664–1650 cm⁻¹ typical of Amida 1, due to carbonyl. In C=C aromatic vibration. H-N refers to collagen, stretching C-N protein peptide ligation. It is confirmed by biochemical biotransformation carried out by the fungal population.