USE OF THE ALLIUM CEPA BIOASSAY AS AN INDICATOR OF CYTOTOXICITY OF PESTICIDES IN VITICULTURE

Authors

  • Renata Sano Lini
  • Eduardo Daniel Righi Dornelles
  • Camilla Yumi Endo
  • Débora Hayane Vidal Pereira
  • Larissa Tiemi Akamine Motomura
  • Mariana Yumi Date
  • Thalita Fontana Manocchi
  • Priscilla de Laet Santana
  • Tânia Cristina Alexandrino Becker
  • Edilson Nobuyoshi Kaneshima
  • Simone Aparecida Galerani Mossini
  • Alice Maria de Souza Kaneshima

DOI:

https://doi.org/10.56238/sevened2026.020-005

Keywords:

Mitotic Index, Biological Assay, Onions, Agrochemical

Abstract

Objectives: To evaluate the cytotoxic effect of pesticides used in grape cultivation in Marialva-Paraná through the Allium cepa bioassay.

Methods: Different concentrations of pesticides were tested: imidacloprid, Metiram+Pyraclostrobin, Metalaxyl-m+Mancozeb and Tetraconazole. The test solutions were prepared based on the recommended doses for grape cultivation and also with variations in these concentrations. Cytotoxicity was assessed by calculating the mitotic index.

Results: Macroscopic analysis showed that, with increasing concentration tested, there was an increase in fragility and a decrease in root size. For all substances tested, there was a significant reduction in the size of the roots compared to the substances with the respective negative controls. Cytotoxicity was confirmed by calculating the mitotic index. Furthermore, during the counting of cells that presented different phases of cell division, some abnormalities were observed in the genetic material indicative of genotoxicity.

Conclusions: This study confirmed the cytotoxicity of all active ingredients tested, and the information presented aims to contribute to the development of additional research to relate cytogenotoxicity in humans.

Downloads

Published

2026-04-19

Issue

Pathways to Health: Innovations and Approaches in Health Sciences - 2nd Edition

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

Lini, R. S., Dornelles, E. D. R., Endo, C. Y., Pereira, D. H. V., Motomura, L. T. A., Date, M. Y., Manocchi, T. F., Santana, P. de L., Becker, T. C. A., Kaneshima, E. N., Mossini, S. A. G., & Kaneshima, A. M. de S. (2026). USE OF THE ALLIUM CEPA BIOASSAY AS AN INDICATOR OF CYTOTOXICITY OF PESTICIDES IN VITICULTURE. Seven Editora, 47-62. https://doi.org/10.56238/sevened2026.020-005