INHIBITORY EFFECT OF THE ETHANOLIC EXTRACT OF Bidens pilosa L. LEAVES ON THE GROWTH OF Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli AND Saccharomyces cerevisiae
DOI:
https://doi.org/10.56238/rcsv16n5-003Keywords:
Plant Extract Production, Antimicrobial Activity, Bidens pilosa LAbstract
The increase in bacterial resistance to conventional antimicrobials has driven the search for new therapeutic alternatives, with natural products standing out as promising sources of bioactive compounds. This study evaluated the antimicrobial potential of the ethanolic extract of Bidens pilosa L. (picão-preto) leaves. The extract was obtained by macerating 10g of leaves in 150mL of 80% ethanol for seven days, filtered, and concentrated in a water bath at 60°C to 20% of the original volume. Antimicrobial activity was evaluated in liquid medium against Saccharomyces cerevisiae and in solid medium by the disk diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Results showed a 19.04% reduction in S. cerevisiae cell viability after exposure to the extract. In solid medium assays, inhibition zones were observed for all microorganisms: S. aureus (21-24 mm), P. aeruginosa (30-33 mm), and E. coli (21-23 mm). Activity against E. coli particularly relevant, considering the characteristic resistance of Gram-negative bacteria. The results confirm the broad-spectrum antimicrobial potential of Bidens pilosa extract, attributed to the presence of secondary metabolites such as flavonoids and phenolic compounds.
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